潮霉素B/Hygromycin B

级别：Ultra pure grade
含量：≥80%

浓度：50mg/ml

Working concentration: 50～1000µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used
LD50：6 mg/kg in mouse （i.v.）；13 mg/kg in guinea pig （i.p.）；63 mg/kg in rat （i.p.）
性状：液体，50 mg/ml, in PBS （phosphate-buffered saline）, sterile-filtered
用途：生化研究。是一种氨基糖苷类抗生素，通过阻断多肽的合成，抑制多肽延长，用于原核和真核细胞的筛选。Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene （hyg or hph）.
保存：2～8℃