Super Script Ⅱ阴性逆转录酶

英文名称：SuperScript II Reverse Transcriptase

级别：BR

来源：Purified from E. coli expressing the pol gene of M-MLV （1,2）, mutagenized to reduce the RNase H activity
浓度：200u/ul

活力定义：One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of eoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly（A）•oligo（dT）12-18 as template•primer （3）.

Unit Reaction Conditions：50 mM Tris-HC1 （pH 8.3）, 40 mM KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly（A）, 0.1 mM oligo（dT）12-18 , 0.1 mg/m1 BSA, and enzyme in 50 µl for 10 min. at 37℃

Performance and Quality Testing：SDS-PAGE purity；endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product

Enzyme Function：RNA dependent DNA Polymerase

Final Product Size：12.3 kb or less

Number of Reactions：10 Reactions

Optimal Reaction Temperature：42℃

Sensitivity：Medium

Template：ssRNA；ssDNA；RNA-DNA Hybrid

产品描述：SuperScript® II Reverse Transcriptase （RT） is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations rather than a deletion in the RNase H active center. Like SuperScript® RT, SuperScript® II RT has reduced RNase H activity. Unlike SuperScript® RT, however, the selective mutations within the RNase H domain maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics, including：Greater first-strand cDNA yields；More full-length cDNA synthesis；Full activity at 42℃
性状：悬浮液
用途：生化研究。
保存：-20℃