英文名称:Glucose-6-phosphate Dehydrogenase;G-6-PD;G-6-P-DH
其他名称:葡萄糖-6-磷酸脱氢酶;6-磷酸葡萄糖脱氢酶
CAS号:9001-40-5
级别:Chromatography purified
分子量:128KDa
活力:≥200 NAD units/mg
活力定义:One unit reduces one micromole of NAD per minute at 37℃,pH 7.8,using glucose-6-phosphate as substrate
Phosphohexose isomerase:≤0.02%
Phosphogluconate dehydrogenase:≤0.003%
Adenylate kinase:≤0.002%
Creatine phosphokinase:≤0.002%
描述:The Leuconostoc GPDH exhibits dual coenzyme specificity,namely NAD and NADP,when assayed under conditions that are optimal for the particular coenzyme,the ratio of observed catalytic activity is NAD/NADP=1.8
性状:悬浮液。Suspension in 2.4M ammonium sulfate。等电点PH4.5
用途:用于生化研究,是红细胞糖代谢中的一种酶,其作用是保持还原型谷胱甘肽和还原型辅酶Ⅱ(NADPH),维持红细胞内的还原能力,防止过氧化氢等对红细胞的损伤。此外,它能催化6-磷酸葡萄糖脱氢反应,可用于酶法检测NAD+ (或NADP+)、6磷酸葡萄糖、磷酸葡萄糖异构酶、磷酸葡萄糖变位酶和己糖激酶。它和己糖激酶联用用于葡萄糖的酶法检测
保存:2~8℃
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